Therefore, a species tree based on data from two different experimental methods yields an inconsistent picture (see Fig. 6A), in which the wanted contributions of the 'species distance' are modulated with the unwanted contributions of the 'methodological distance' originating from sampling biases.
Of six isolates identified as C. lusitaniae by molecular methods, one showed an inconsistent profile with the biochemical test.
Our simulations also show that BI recovers an incorrect phylogeny with increasing support as the amount of data increases, as expected for an inconsistent method but not a consistent one.
Since the bargaining power has to lie within the unit interval, ordinary least squares is an inconsistent estimation method and censored regression techniques (such as a Tobit regression) have to be employed.
Thus, given two data sets, one of which has parameters prone to long-branch attraction [ 46, 47], the choice of an inconsistent phylogenetic method to analyse both data sets may produce different trees.
However, the interpretation of these results is complicated by the fact that Suzuki et al. (2001) calculated additivity expectations by multiplication of unscaled effect measures, a method inconsistent with independent action.
In case of the LBA artefact, tree reconstruction methods are inconsistent, i.e. they converge towards an incorrect solution as more data are considered.
Our study demonstrated that FA values measured with FT methods and ROI methods were inconsistent (r = 0.528), while ADC values had a relatively good consistency (r = 0.822).
However, these assay methods yielded inconsistent results, likely because of interfering substances and other peroxidases present in tissue extracts.
Only one study also used physiological methods with inconsistent results in subjective and objective data.
