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However, higher accuracy methods also suffer from errors, those are often filtered by low order methods.
Iterative methods also suffer from low bias in low-count situations due to the non-negativity constraint which is typical for Maximum Likelihood Expectation Maximization (MLEM) (i.e, image voxels never have negative values due to the multiplicative nature of MLEM and the fact that the initial image is not negative).
These classic methods also suffer from the problem of incomplete filling of large and/or complex neuronal processes.
Indirect methods also suffer from limited digital resolution along the large C chemical shift dimension.
This makes the algorithm can merely applied to well-annotated species while all other methods also suffer the same issue more or less.
These methods also suffer from incomplete coverage and uneven amplification of the genomic loci of several orders of magnitude, with 10-2 ~ 10-4 and 10-3 ~ 10-6 fold amplification biases for PEP and DOP-PCR methods, respectively [ 50].
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But the method also suffers from "a kind of Heisenberg uncertainty problem," he adds.
Additionally, this method also suffers with scattered edge pixels of moving objects.
This method also suffers from the weak reflectivity of the moving target and strong background.
This method also suffers from the same drawbacks: it is compression algorithm specific and cannot survive transcoding.
Although SPT has opened the window to increasing the resolution of structural biology in situ by averaging repeating features in cellular tomograms, this method also suffers from significant limitations.
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