Application of this method allows whole genome studies from samples where material or yields are limiting.
However, solid phase 'next generation' sequencing methods should soon allow whole genome sequencing to be performed on population samples [50].
These two methods allow the detection of whole chromosome aneuploidy for chromosomes 13, 18, 21, X, and Y.
Whole-genome methods allow for background genetic effects by incorporating all markers, and thus all the associations between marker and trait are estimated simultaneously.
Because reduced representation bisulfite sequencing (RRBS) covers only 5%100% CpGs with bias toward high-CpG-density regions (Guo et al., 2014; Smith et al., 2014), we adopted the post-bisulfite adaptor tagging (PBAT) method, which allows whole-genome bisulfite sequencing with picogram level of input DNA (Kobayashi et al., 2013; Miura et al., 2012).
Whole-genome prediction methods allow predicting complex traits, irrespective of knowledge of their molecular basis.
Additionally, our method is highly scalable, allowing whole computer clusters to be fully utilized for large-scale multiple genome comparison.
The development of new computational methods allows for the whole-network modelling of metabolism and conduction of compelling and testable predictions even without many parameters.
In order to find out how widely is this phenomenon spread among other mammals and also to identify species which would complement the rodent model system for studying this problem, we need to analyze carefully the incidence of this phenomenon in other mammals with longer lifespan using methods allowing for analysis of the whole set of chromosomes, such as CGH.
Methods allowing complete extraction of organic contaminants from whole water samples are required.
