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To modify the manuscript, we have added the following sentences in Materials and methods, "Adjustment of Mutation Frequency": "To consider tumor content we performed CD19/CD5 FACS analysis to quantify the fraction of tumor cells".
Particularly, the 95% confidence interval of MAF at 0.5%, 2.5%, 5%, and 25% are used to estimate upper and lower bound of MCF with the formula described in Materials and methods, "Adjustment of Mutation Allele Frequency", and Figure 1 figure supplement 1.
Also we have added the following sentence in Materials and methods, "Adjustment of Mutation Frequency": "MCF of a copy number abnormality (i.e. T12, del11q, del13q_x1, del13q_x2, del17p, delBIRC3, delRB1_x1, delRB1_x2) was calculated by FISH analysis divided by the fraction of CD19/CD5 cells based on FACS, correcting for the tumor purity".
Similar(57)
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Methods: Data sources.
Methods: IRB approval was obtained.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com