With the introduction of these methods, the average time needed for a reliable and validated identification ranged from 6 h to 18 h and in the last few years, sequence analysis of small-subunit rRNAs or selected genes by PCR methods has complemented the biochemical methods, additionally decreasing throughput time and becoming in several cases the gold standard [1].
Detection and identification of borrelia was performed by molecular methods and additionally in the prospective study by isolation and genotyping (MluI-LRFP and MLST).
Additionally, In-doped ZnO nanostructures have been grown by the hydrothermal method.
Additionally, in methods described in detail elsewhere [13], standard deviations in length from the mean domain size were also calculated for each member using standard formula and averaged for the entire superfamily.
The method is additionally advantageous in that reference data only have to be prepared once and from a user's point of view only the unknown sample has to be derivatized for use in CASPER.
Additionally, in RP methods, lipid elution requires longer gradients and can span a wide range of the chromatographic run, which could affect the detection of smaller molecules.
Additionally, in the method proposed by Matsu'ura and Hasegawa ([1987]), the parameters are constrained to be initial values as a priori information.
Additionally in this method, all SNPs were used to calculate PCs.
In the workbook presentation of the tool [ 26], each method was additionally described briefly in a page per method regarding its typical functions, purposes and example application areas.
Additionally, in the latter method, which treats the atomic partial charge as a fourth dimension in describing the atom position vector, charge scaling must be made, and the way adopted for that was purely a matter of trial-and-error to obtain the best enrichment factors.
