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Even with direct methods, a major issue lies in the initialization procedure.
Compared to alternative molecular methods, a major advantage is that results are unbiased (a list of nucleotides) and highly reproducible, thus enabling the comparison of data from different laboratories and time periods.
Despite all this progress in imaging methods, a major problem remains: the lifetime of every fluorophore is limited by photochemical destruction, generally called photobleaching.
Despite the obvious appeal of consensus between different analytical methods, a major caveat in using polling strategies for mutation assessment is that these approaches are prone to both systematic and sampling errors.
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Compared with parametric estimation method, a major advantage of the kernel method is that it can be used for the PDF without explicit expression.
For the DNA samples extracted with the CLU method, a major high-intensity, discrete band with a high molecular weight of approximately 10 kb and limited smearing of degraded DNA were also observed (Fig. 3).
However, the Fourier transform method requires only a single run, providing major savings in computational time as compared to the Monte Carlo method, a major advantage for analysis of power systems whether operating under full or partial load.
Given the limitations associated with each method, a major strength of this analysis was the use of multiple methods to provide a range of estimates.
Although a wide spectrum of QM charge calculation methods are available, all the methods have a major limitation they are very time-demanding.
Distance matrix methods constitute a major family of phylogenetic estimation methods, and the minimum evolution (ME) principle (aiming at recovering the phylogeny with shortest length) is one of the most commonly used optimality criteria for estimating phylogenetic trees.
Modern stunning methods are a major advance in pursuit of the humane ideal (that also lies behind various ancient traditional slaughter methods) of avoidance or minimising the risk of causing pain or other unpleasant feelings at the time of slaughter.
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