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In order to verify this methodology, we probe here the effect of EGCG in combination with the adjuvant digitonin as a membrane permeabilising agent on Plasmodium sporozoite motility and survival.
To demonstrate this methodology, we probe the in vivo architecture of the γ-Tubulin Ring.
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Using this methodology, we identified 586 probe sets representing 484 genes across EG1 with differential expression (DE) higher than ±1.5-fold in more than 50% of individual pair-wise comparisons, and 1423 probe sets representing 1087 genes across EG2, again with the same criteria of DE > ±1.5 in > 50% of experiments, whereas 180 probe sets representing 155 genes were present in both data sets.
To further determine the effect of probe redundancy in our APEX methodology, we used LDA to reanalyze both data sets (original and 50-plex) but using non-redundant and partially-redundant probe-specific data [Additional files 8, 9 and 10].
The ChIP-on-chip methodology we used only detects promoters, since only probes for the promoters are on the tiling arrays that we used.
By employing this conservative methodology, we reduce the risk of inappropriately comparing data from probes designed to detect different transcripts or genes despite having a similar annotation.
We have optimized methodology to probe human protein microarrays with full-length RNA molecules and have identified 137 RNA-protein interactions specific for 10 coding and non-coding RNAs.
In the process of addressing this particular question, we also wanted to establish a methodology to probe the electrodynamics of functional connectivity during childhood.
We probed but lacked bravery.
Activity-based protein profiling represents a powerful methodology to probe the activity state of enzymes under various physiological conditions.
Whatever the mechanism for haem demetallation and degradation in our selenium-substituted structure, this study also provides a unique methodology to probe the role of axial ligands to haem in bacterioferritin protein family.
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CEO of Professional Science Editing for Scientists @ prosciediting.com