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Using this methodology, we detected that the average gap size was around 500 ± 300 bp, corroborating with LW average gap size estimative (413 bp).
Applying our methodology, we detected small amounts of detergent-insoluble WT hSOD1 in spinal cords of older Gurney WT SOD1 mice but not in L76WT mice (Fig. 2).
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Moreover, with the same methodology (ELISPOT assay), we detected a significant decrease of the specific anti-tuberculosis T-cell response in patients after 12 weeks of anti-TNF therapy [ 31].
The ChIP-on-chip methodology we used only detects promoters, since only probes for the promoters are on the tiling arrays that we used.
Using our methodology we were able to detect this mutation among one of the 16 samples of the pool, which further support the validity of our method in terms of sequencing capacity, genotyping and filtering methods (Supplementary Fig. S3).
Although no quantitative inferences could be made, using this simple methodology we were able to detect the eight COG transcripts in 20 human tissues thereby confirming their ubiquitous nature.
Using last-generation SD-OCT methodology, we were able to detect a mild reduction in the total macular thickness, and manual segmentation revealed a unique pattern of retinal pathology: while we found a reduction in the retinal nerve fiber layer, suggesting axonal degeneration in ALS, and a marked thinning of the INL, the corresponding GCIP was not altered.
In fact, what really defines this zero-state is the high concentration of BCL2T, as evident from the green points in Figure 7, panel D. Summarizing, following our methodology we were able to detect bistabilities in the apoptosis model and to characterize different model evolutions in terms of parameter changes, reaching conclusions similar to those discussed in [ 28].
METHODOLOGY/PRINCIPAL FINDINGS: We detect significant differences of energy features at experimentally supported human miRNA target sites and at genome-wide sites of AGO protein interaction.
Using this methodology we have been able to detect various biochemical mediators including nerve growth factor (NGF), prostaglandin E2 (PG-E2) substance P, IL-1β, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNFα, INFγ, G-CSF, GM-CSF, MCP-1 and MIP-1β.
The methodology we present here can better detect the solid soil and pore spaces on CT images, enabling the generation of better 2D 3D representations of pore structures from segmented 2D images.
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