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Laboratory medicine should make a great effort in todays community work to align and standardize the methodology of endogenous biomolecule determination.
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Understanding the in vitro routes of biological processing should help guide the development of analytical methodologies for the detection of endogenous adducts.
The wide variation in the GH half-lives reported in the literature has been explained by the use of different methodologies, and interference by the secretion of endogenous GH. 23, 25 A limitation of the current study therefore is that endogenous GH secretion was not suppressed.
This methodology was applied on a substantial number of endogenous hormones, oral contraceptives, and a wide range of environmental pollutants known to be present in human serum.
The same small molecule dimerization methodology was recently adopted to demonstrate inducible control of endogenous genes.
The down-regulation of endogenous SLPI expression in AGS cells by siRNA methodology did not affect the Progranulin expression independent of the infection by H. pylori.
The novel methodology determines endogenous modal shares by incorporating variables related to the level-of-service (LoS) of modes and consumers' modal perception within the E4 modeling framework.
The direct detection of endogenous DSBs in individual cells has been difficult because the leading methodology, single cell electrophoresis (comet) assay [ 29], lacks the desired sensitivity.
Thus, the methodologies developed in this work allow for the systematic prediction, detection, and manipulation of endogenous signaling gradients in vitro along similar mechanisms found in vivo in order to regulate cell fate.
However, current methodologies are limited in terms of the capacity to isolate and expand a sufficient quantity of endogenous antigen-specific Tregs for therapeutic intervention.
Thus, in the context of discrepant evidence about the true scale of endogenous mammalian regeneration, it is worth reviewing potential sources of bias inherent to all existent methodologies that are used to study cell fate.
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