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Our methodology for mitochondrial isolation is shown [27].
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Previous methodologies for studying mitochondrial aging have severe limitations including a lack of spatial resolution and live-cell applicability. 2 We present a novel approach based on a time-sensitive fluorescent protein that is targeted to the mitochondrial matrix (MitoTimer).
(32) The availability of labeled CoASH will also facilitate the development of methodology for the quantification of this important mitochondrial thiol and its oxidized mixed disulfide derivatives such as CoA−glutathione.
With regard to the latter, novel mitochondrial gene replacement methodologies in stem cells may aid with the developing animal models for mitochondrial diseases in the future.
There are no cures for mitochondrial disorders.
They were sent to the US for mitochondrial DNA testing, which focuses on the genetic makeup of hair cells.
Mitochondrial biogenesis was not responsible for mitochondrial superabundance, whereas mitophagy was suppressed despite impaired mitochondrial proteostasis.
The purified mitochondrial fraction was used for mitochondrial respiration and mitochondrial membrane potential measurements.
Our protocol for mitochondrial isolation is illustrated.
Mitochondrial-localizing RFP (Mt-RFP) was used as a marker for mitochondrial localization.
These electrodes are being used for mitochondrial membrane potential studies.
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