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The same group also applied this methodology for labeling peptides that had been site selectively functionalized with an azide.
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Therefore, we sought to develop a general methodology for labelling PPMOs that utilizes commercially available, unfunctionalized PMOs and which would retain the label close to the PMO in the event of peptide proteolysis in vivo or intracellularly.
The use of Biophotonic Sensing Cells (BICELLs) based on micro-patterned photonic architectures has been recently proven as an efficient methodology for label-free biosensing by using optical interrogation.
These include RNA labeling methodologies [ 14], detection calling [ 15], quality control checks for labeling [ 16] and CEL file assessment [ 17], and analytical methodologies [ 18, 19].
This intriguing methodology can be easily transferred into a commercial kit, making it readily available for labeling biomolecules for clinical applications.
Things for labeling.
In summary, MTRIP labeling of viral RNA has the potential to become a general methodology for the labeling and study of many different RNA viruses.
The implication of this development is that MTRIP labeling of viral RNA during virus assembly has the potential to become a general methodology for the labeling and study of many important RNA viruses.
"This is not the kind of DNA people are used to hearing about," explains Martin Tenniswood, the molecular biologist who came up with the methodology for the labels.
We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide).
The majority of the developed methodologies rely on the use of [F]fluorosugars for labelling.
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