SPMNPs based subcellular fractionation is an effective and simple methodology for isolating plasma membrane, early endosomes, late endosomes and lysosomes in any given eukaryotic cell [28].
We developed methodology for isolating RNA from the buffy coats and conducted omic analyses.
We took advantage of next-generation sequencing technology to develop and internally validate a new methodology for isolating integration sites and estimating the number of cells in each HTLV-1-infected clone (clone size).
A lack of suitable methodology for isolating, extracting and sequencing DNA from individual Symbiodinium cells has meant that intra-genomic variation in this genus has remained virtually unexplored [ 50].
Secondly, the study methodology for isolating M. pneumoniae used polymerase chain reaction (PCR) from bronchoalveolar lavage and throat swab samples, which were available for almost all patients, as well as sputum samples, which were available in only approximately 40%% of patients.
Most of the recognized methodologies for isolating CTCs and DTCs could be classified as cell filtration, antibody capture and enrichment.
The goal of this presentation is to provide a unified methodology for detecting, isolating and accommodating both abrupt and incipient faults in a class of nonlinear dynamic systems.
In addition, while BOX-PCR gels can be visualised directly for assessing small numbers of isolates, our methodology for electronic analysis of larger isolate numbers requires purchase of software modules.
Details of the study design, including the selection of patients and the methodology for the identification of isolates and their storage in the PROTEKT study has been described previously [ 24].
Methodology to isolate single neurons for RNA analysis was recently described by our laboratory (Akhmedov et al., 2013, Journal of Visualized Experiments, Accepted).
