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Using 3-dimensional echocardiography in conjunction with novel geometric modeling and rendering techniques, we have developed a high-resolution, quantitative, 3-dimensional methodology for imaging the human mitral valve.
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Current methodologies for imaging the biliary system during cholecystectomy are cumbersome and do not eliminate the risk of bile duct injury.
Research on image data management and analysis plays a key role in improving the performance of protocols, software-based analysis and further methodologies for imaging biobanks and the development and validation of imaging biomarkers.
Currently available methodologies for imaging live small model organisms, such as Drosophila or Caenorhabditis elegans are based mainly on differential interference contrast (DIC) and confocal epi-fluorescence microscopy.
That report provided only a limited description of the methodology used for imaging the marginal artery, and this perhaps reinforces the view of the current authors about the need for careful position and patient description for the establishment of baseline reference values.
The reported methodology could be extended for imaging composite rough surfaces when contrast is based on mechanical properties, adhesion and electrical conductivity.
This methodology is particularly attractive for imaging studies that make use of the small tip size.
Based on the instrumentation and methodology described above, we developed a dedicated workflow for imaging metabolite distribution in single cells (Schober et al. 2012b).
In summary, we provide protocols for novel, time-saving and robust methodology convenient for imaging of migration and chemotaxis of B lymphocytes.
We thank A McCauley for imaging assistance.
Sections were mounted in mowiol for imaging.
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