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For example, the whole primer sets for amplifying the entire circular mtDNA for Chimpanzees (Pan troglodytes; accession no. NC 001643) [52] and Bonobo (Pan paniscus; accession no. GU189657) [53] are successfully mined using our proposed methodology (data not shown).
Our results were largely insensitive to the method of calculating the monthly mean CO2 or to detrending methodology (data not shown).
However, no observable fluorescent complex formed when any of the mutants were coexpressed with AKT as assayed by the PCA methodology (data not shown).
An excessive number of SCID mice died following the glioma injections due to brain hemorrhage; this complication or high death rate was not observed with NU-FoxnNu or NIHIII mice using the same brain injection methodology (data not shown).
The mutations in the other four genes (ftsK, stfE, C0362 and rsxC) reported by WG and the five other genes (mdlB, yagN, ydfN, ykfA and yagG) reported by RN (Tables S1 and S2 presented in Additional file 1 and Additional file 2), could not be confirmed by the Sanger methodology (data not shown).
For the metagenome analysis, we have taken account for all the oocyte-specific genes identified so far by the in silico methodology (data not shown), including well known genes (Mater, Zar1), as well as the 12 genes of the oogenesin family [ 7], and the 6 genes similar to NALP5/MATER [ 8] that we have previously identified.
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Ideally we would have separated the two mucins but this was not possible using current separation methodologies (data not shown).
Most of the proposed indicators for SDG5 are classified as tier two (methodology exists, data not easily available) or tier three (methodology needs to be developed).
A NASA spokesman, David Mould, said Monday that the purpose of the study was "to develop methodologies" for analyzing data, not to find what the data would show.
We also applied the same methodology for the autosomes (data not shown).
To determine what role this last factor might have, we examined survival curves and performed additional analyses using death as a competing risk (data not shown, methodology described in [ 32]).
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