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Differences in transamidating activity measurement methodology, cell types and stable overexpression in comparison to transient transfection of TG2 may explain the lack of transamidating activation in response to hyperosmotic stress.
In this last methodology, cell death due to a loss in cell membrane integrity of drug-treated L. amazonensis was assessed by flow cytometry measuring the level of propidium iodide uptake into damaged promastigotes.
Additional rheological analysis of hydrogel, SAXS curves, images of in vivo methodology, cell cytocompatibility.
Bioorthogonal chemistry has evolved to be a rather unusual field in that it brings together traditional mechanistic chemistry, reaction methodology, cell biology, and biomedicine.
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Using this methodology, cells or organisms are first treated with a monosaccharide building block bearing a chemically reactive tag.
After 48 hours of exposure, the cytotoxicity was measured by the SRB methodology: cells were fixed by adding 50 μl of cold 50% (w/v) trichloroacetic acid (TCA), and incubating for 60 minutes at 4°C.
By using the microfluidic methodology, cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence where agonist (ionomycin) evoked intracellular Ca2+ fluxes and the cell's real time spectral response was recorded.
Cell growth was monitored by either colorimetric (optical density at the wavelength of 600 nm) or gravimetric methodologies (cell dry weight).
The direct detection of endogenous DSBs in individual cells has been difficult because the leading methodology, single cell electrophoresis (comet) assay [ 29], lacks the desired sensitivity.
A new methodology for cell formation, which considers the scheduling and operational aspects in cell design under demand changes, is introduced in Phase II.
The methodology for cell competition is based on bacterial batch culture competition models [ 46], in which cells are maintained in logarithmic growth and serially passaged.
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