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The mutant strains were constructed using the lambda Red recombinase methodology, as previously described [18].
The latter was defined as the percentage of fibres having a diameter larger than 30 µm by measuring 4000 individual fibre snippets per sample using the Optical Fibre Diameter Analyzer (OFDA) methodology as previously described [36].
Thus, we examined the molecular similarity between reclassified MFH samples and their predicted corresponding STS subtypes from different datasets using the Subclass Mapping (SubMap) methodology as previously described [14] (Gene Pattern Software, Version 3.0, Broad Institute, details in Data S1).
P-glycoprotein (Pgp) protein and function was measured using flow cytometric methodology as previously described [ 23].
Methacholine challenge was performed according to a standardised methodology as previously described [ 11].
The survey methodology, as previously described (4), comprised self-administered questionnaires, physical measurements, and laboratory tests.
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The criteria for the construction were the same for the four methodologies, as previously described.
Genotypes were determined using PCR-based methodologies as previously described (Zhu et al., 2002; Proctor et al., 2005).
The secondary swine anti-rabbit horseradish peroxidase (HRP -conjugated antibody, as well as nonimmune rabbit serum were obtained from DAKO (Cambridge, UK) ImmunoHRP -conjugatedtantibodyor caseolin-1 wellcasried out usinonimmunerabbithodologieserumpreviously described (Campbell et al, 2002).
The team developed a WoE assessment methodology as described previously.
We detected HPV DNA in cervical samples by PCR-based methodology as described previously.
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