BMPs have been thoroughly studied in the differentiation of ASCs by methodologies including addition of recombinant protein, addition of neutralizing antibodies, viral over expression and siRNA [22], [32], [41], [42].
Early clinical trials are no exception, with efforts to improve the eventual success rate of late stage trials through evolving phase I trial methodologies, the addition of extensive pharmacodynamic studies, and early adoption of patient selection strategies.
(The survey results can't be compared with prior years' surveys because of a change in methodology, including the addition of cellphones to the survey sample).
The substrate did not only enhance the protection of probiotic bacteria; but it was also a more complex methodology than direct addition of lyophilized culture.
Several studies dealt with TPOMW detoxification through the development of the appropriate solid-state fermentation methodologies and the addition of suitable co-composting materials for coping with the particular structure of this effluent [ 3, 4].
Titled the Renal Sympathetic Denervation in Patients with Treatment Resistant Hypertension or Symplicity HTN-2, the trial essentially mirrored its brother trial in terms of methodology but with the addition of a randomised (but nonblinded) control arm.
This methodology involved the diastereoselective addition of a metalloenamine 6 to an aldehyde followed by diastereoselective imine reduction (Scheme 1).[ 8] We proposed that the reaction of 7 with ethyl dimethylpyruvate could establish the required stereogenic center of the tertiary alcohol.
Subsequent to this dataset, in an attempt to increase the population of genomic end viral reads, the 454 sample preparation methodology was modified by the addition of 1pmol of N165-146 and LRACEF2 at the hexamer cDNA synthesis stage, to enhance the cDNA population containing the genomic termini.
Despite improvements in staging, surgical methodologies, chemotherapy regimens and the addition of adjuvant therapies, 30 to 50% of patients with resectable NSCLC suffer relapse and die within 5 years [ 15- 17].
Given CBPR does not outline a specific and rigorous methodology, however, Windsor (2013) proposes the addition of mixed scientific methods to ensure adequate rigor in the production of new knowledge.
