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The experimental determination of the structure corresponding to the consensus sequence using the above mentioned methodologies would require the (chemical or enzymatic) synthesis of a real homogeneous population of RNA molecules harboring a sequence identical to the consensus of the population.
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This shift in methodology would require some significant design changes in both the memory chips and logic chips.
However, this study is restricted to the agronomic and environmental conditions of the site, and the methodology would require validation and potential adjustment in other locations and conditions.
In a comparative analysis of 31 countries as the one presented in this paper, such methodology would require, among other things, corroborating the estimation of the costs with a focus group of disabled individuals in 31 countries.
Performing functional analyses of miRNA-mRNA interactions using standard methodology would require measuring global expression of mRNA and miRNA using two different arrays or RNA-sequencing experiments.
As a result, substantial improvement in methodology would require concerted, long-term actions with interdisciplinary collaborations among scientists, engineers, medical researchers, and practitioners.
For example, development of a sequencing test for a new gene in a laboratory with extensive experience in sequencing may simply be a case of primer design, whereas setting up an entirely new methodology would require much more extensive investigation.
Application of such a staged treatment methodology, however, would require a thorough understanding of both physical chemical treatment technologies and source-zone bio-remediation.
We would like to stress that we did not apply the full weighted CC and TOM methodology since it would require the construction of the whole network which was beyond the aims of our study.
A fair comparison of the methodologies would therefore require the application of each methodology to each set of primary resources, and a systematic investigation of the differences between the resulting reconciliations.
But our methodology is perfectible and would require a randomized control tria.
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