Comparing to Sanger sequencing, significantly more consensus mutations were detected by the DxS kit (P = 0.0139), high resolution melting (P = 0.0004), and SNaPshot (P = 0.00001), but no statistically significant differences were found among the three methodologies with higher sensitivity.
Among many challenges this application holds; development of more efficient and robust statistical methodologies with higher power to detect an association with a single marker has been one of the most important statistical issues, given that effects of individual markers are usually characterized as being small to moderate.
The introduction of PCR-based methodologies with higher sensitivity, to overcome the gaps of traditional parasitological approaches (xenodiagnosis and hemoculture), offered important contributions in the context of Chagas disease cure control and still supports an improved identification of the parasite persistence after etiological treatment.
In addition, data of the DHS are widely perceived to be of high quality, as they were based on sound sampling methodology with high response rate.
In addition, data of the DHS are widely perceived to be of high quality, as they were based on sound sampling methodology with high response rates of about 98%.
NOx storage and reduction (NSR) catalysts containing Pt, Ba and Fe were studied as a function of reaction conditions and catalyst composition using response surface methodology combined with high-throughput experimentation.
Reports are concise manuscripts that highlight a specific finding, model or methodology with a high impact on the field of molecular medicine.
Simulation results on a rudimentary 3-bus system and several IEEE standard test systems fully illustrate that the proposed methodology is effective with high efficiency.
Detection of high screening BP by self-screening is similar to that of more resource-intensive methodologies to identify participants with high BP, but cannot currently be recommended due to a paucity of data on this methodology.
