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Before the advent of NGS sequencing, four different methodologies were already available to enrich DNA targets of interest: PCR-based enrichment, microarray- or liquid-based hybridization, restriction enzyme-based enrichment, and physical isolation of mRNA [ 11].
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The league table methodologies are already the subject of debate and academic comment.
Probe enrichment methodologies are already available for modern human, mouse, dog and cattle DNA re-sequencing projects, which are applied either to study specific genomic regions genetically related to diseases or to analyze a whole exome (i.e. coding exonic DNA) [ 121, 122].
Agile software development methodology is already used for the development of mobile software for the reason that the agile methodology is capable of covering some critical features of the MCE: Various requirements.
Because the participating organizations already apply rehabilitation principles and specifically the CARe methodology is already part of the participating organizations no informed consent is needed for the group randomization and the receiving of care according to the CARe methodology.
Furthermore, it paves the way for other health intervention activities and therefore in general serves to improve the health of the people.'" So by 1991 community based methodology was already evolving in Uganda and its use for other health activities was foreseen.
This applied methodology was already used in a study by De Morton in 2010 [ 13] dealing with the validity and reliability of the DEMMI in a geriatric rehabilitation setting and in a study by Stubbs in 2014 [ 36] about the FIM in patients with acquired brain injury.
The demands for reliable and fast methodologies have been already increased in dairy industry.
When doing so, however, it is important to think about how these approaches can be combined with low-tech methodologies, which are already known to be effective.
The design procedure is derived in the state-space framework, in contrast to frequency-based design methodologies, which are already studied in the literature.
As we already discussed in Section 2, we are certainly not the first to measure skill mismatch and a variety of methodologies have been already proposed in the literature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com