Additional details of the robotics-integrated ARQiv system and the methodologies we apply in pursuing whole-organism HTS in zebrafish larvae can be found here: (White et al., 2015).
In order to test the strength of the proposed methodology we applied it to a case study in the above-tree-line glacial cirque of Mount Prado (Northern Apennines, N Italy).
In addition to this section, this paper has the following sections: Section 2 describes the case description with: the description of the methodology we applied in this research, and the description of the theoretical basis of the techniques and notation used in the analysis and design phases.
In order to test the performance of the proposed methodology, we applied it to the South American region with data obtained by RAPEAS (Red Argentina para el Estudio de la Atmósfera Superior, i.e. Argentine Network for the Study of the Upper Atmosphere) during the years 1958 2009.
To validate the specificity and sensitivity of this methodology, we applied the screening assay to cellular conditions and proteins of interest known to regulate macropinocytosis.
In order to determine whether the disease signal could be validated using an alternative methodology, we applied the method of Cheadle et al [32] to validate across chip platforms.
In order to ascertain the sensitivity of the methodology, we applied the system to various simulated, artificial increases in the number of disease presentations or 'hotspots'hotspots
In order to illustrate the application of our methodology, we applied the gains derived from normal animals to two treatments known to alter ERG outcomes.
Since the IV methodology we applied is based on the one-stage approach, we assessed the reproducibility and the equivalence of the approaches using our previously reported results [ 39, 40].
In this context, the innovative methodology we applied provides a repository of patient and nurse behaviors that is discordant to previous reports claiming >95% reliability of monthly medication reviews and record validation [ 19].
