The methodology to dissect the genetics of complex traits is still developing.
The ability to model cancer in the mouse has provided a robust methodology to dissect the molecular etiology of cancer.
In recent years, with the availability of molecular markers to develop well-saturated genetic maps and statistical methodology to dissect complex traits, mapping of quantitative trait loci (QTLs) has proved to be an effective approach to study the genetic architecture of quantitative traits.
We applied statistical methodology to dissect causal relationships between single nucleotide polymorphisms, metabolite concentrations, and serum lipid traits, focusing on 95 genetic loci reproducibly associated with the four main serum lipids (total-, low-density lipoprotein-, and high-density lipoprotein- cholesterol and triglycerides).
In order to improve the sensitivity of the methodologies used to dissect the genetic networks underlying dilated cardiomyopathy, we built a dual-regime OCT instrument dedicated for the in situ measurement of multiple cardiac parameters in the Drosophila larval heart.
Genetic methodologies are also increasingly used to dissect molecular components of P. falciparum pathogenesis, including processes of host-cell invasion, protein trafficking inside the infected host cells, virulence, and immune evasion.
This systems/network pharmacology methodology has been successfully applied to dissect basic TCM theories such as yin-yang theory [9], qi-blood [5], herbal synergy [10, 11], as well as to develop new drugs [7].
Second, multiple methodologies were applied to the detected SNPs to dissect genomic features of the pig breeds, revealing obvious signals of genetic differentiation among the breeds.
In conclusion, the in situ cell extraction assay is a powerful cell-based methodology to evaluate not only bromodomain inhibition, but also to dissect the role/contribution of different domains in chromatin binding and to provide critical insights into both drug-target discovery and basic science.
Knockin methodology, coupled with gene knockouts and specific pharmacological inhibitors, is a powerful approach to dissect the physiological roles of individual modification at given sties on a given protein.
