The sorption profile of these compounds was modelled using experimental design methodology to confirm the first-order kinetics.
We applied a similar methodology to confirm that the distribution of HI orthologues did indeed differ from the set of non-HI orthologues (data not shown).
The results clearly demonstrated that the gene encoding this protein was positively regulated at the transcriptional level, confirming the results of the proteome analysis, and also showing the utility of this methodology to confirm proteome results (6).
Furthermore, this study adds to the previous work by Marcelino and colleagues [ 28], who applied the same methodology to confirm infection by the L. braziliensis species complex in a sample of Rattus norvegicus.
Then in 2013, a study with a different methodology seemed to confirm the effect.
As would be expected, the sensitivity of the laboratory methodology used to confirm infection affects the estimates of vaccine efficacy.
A second limitation of the present study could be the methodology used to confirm the prediction of PET.
The strengths of this work are the large sample size, the inclusion of separate community-based, emergency department and inpatient groups, and the rigourous methodology used to confirm which subjects screening positive for diabetes truly had previously unknown diabetes.
Thus, future studies must demonstrate homogeneous methodologies to statistically confirm the clinical evidence.
This would suggest that those higher in hardiness may have fostered SRG by mobilising their social support for emotional reasons (e.g., moral support, sympathy or understanding) and having the ability to construe their injury in positive terms; however, more rigorous methodologies are needed to confirm or refute these observations.
