We have proposed a methodology of cell seeding into 3-D porous scaffolds using magnetic force and magnetite nanoparticles, which we term Mag-seeding.
The general methodology of cell-SELEX is very similar to traditional SELEX, but fluorescence-activated cell sorting (FACS) can be utilized to achieve a very high level of separation of MRE bound and unbound cell targets.
At variance with other methodologies, the process of cell isolation is not an issue with this technique because the measurements are conducted at the single-cell level.
Using this methodology, shrinkage of cells has been found to be very similar to water loss in intracellular phase (48%).
The advantages of an in ovo model compared to animal models included simple methodology of tumor cell implantation, economical reasons, and high survival rate of embryos.
We have used the classical methodologies of ionomycin treatment, cell surface LAMP1 immunostaining and exocytic release of cathepsin D and hexosaminidase, to demonstrate that mouse epiphyseal chondrocytes can undergo Ca2+-dependent lysosomal exocytosis.
Modification of conventional methodologies to include filtration of cell suspensions, use of "proliferation control" and "cytotoxicity control" cultures as well as vital staining were found to be essential for the valid assessment of primary soft agar cultures in our laboratory.
ES culture was performed on feeder layer, and further electroporation and handling was also performed according to the methodology employed at Dept of Cell Biology, Albert Einstein College of Medicine, and according to Wakayama et al. [21].
ES culture was performed on feeder layer, and further electroporation and handling was also performed according to the methodology employed at Dept of Cell Biology, Albert Einstein College of Medicine, and according to Wakayama et al. In particular, after electroporation, ES cells were re-plated in 55 cm2 dishes and grown until visible clones appeared.
It took almost a century and many devoted investigators until the principles of this scientific methodology, enabling functional analysis of cell and tissue structure by rendering molecules and molecular processes accessible to microscopic detection, became generally accepted.
