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One alternative for increasing the specificity and sensibility of detecting true positives of remote homologous sequences is using three-dimensional (3D) methodologies of alignment and searching.
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Fig. 14 Schematic diagrams presenting a pre-etching and alignment accessory pattern on a wafer and b the methodology of subsequent mask alignment using the technique proposed by Chang and Huang [107] Reprinted from [107], © 2005 Springer, reproduced with permission.
Given the importance of precise alignment as discussed above, it is very important to understand the role of alignment as well as the methodologies to ensure precise mask alignment.
SK contributed to the concept of alignment of expression profiles, invented and implemented primary methodology and primarily wrote the manuscript.
In the third step (table 3), we divided the sequences by the quality of alignment into those for which the alignment was judged to be unambiguous by the HoT methodology (Landan and Graur 2007) and those that contained alignment ambiguities.
Or out of alignment".
"I'm all out of alignment".
"Well out of alignment there," he pronounces.
Also, high winds can knock the dishes out of alignment.
Something is out of alignment, to be sure.
Lack of alignment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com