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With the development of deep sequencing methodologies, it has become important to construct site saturation mutant (SSM) libraries in which every nucleotide/codon in a gene is individually randomized.
However, based on available methodologies, it has been challenging to divide genes into classes depending on functional knowledge and to extract sets of uncharacterized genes from the genome.
By simultaneous measurements with other methodologies, it has been shown that the [oxy-Hb] change measured by NIRS correlates with the rCBF change in 15H2O PET [33] and the blood oxygenation level-dependent [34] signal in fMRI [35].
Combined with the low power associated with standard detection methodologies, it has been difficult to assess rates of paternal leakage in natural populations [ 13].
Finally, by using build-a-genome methodologies, it has been demonstrated that synthetic DNA segments can be assembled (Dymond et al. 2009, 2011).
By giving them 'voice' (through interviews and other 'participative' methodologies), it has been demonstrated how these recruited youths themselves often stress having joined the armed force or group out of their own free will.
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His work has not only broken new ground in methodology, it has had a substantial influence on applied theory.
To verify the methodology it has been successfully applied to a Spanish town.
While this is a robust methodology, it has some limitations seen in other BLAST-based analyses in that homology assignment might be affected by gene fusions, fissions, exon shuffling, lateral gene transfer, or presence of repetitive motifs9.
By means of the life cycle assessment (LCA) methodology, it has been possible to show that the greatest environmental impact occurs during the operational phase (80 90%).
Despite a tremendous development during the last decades, both as regards computer power and methodology, it has remained impossible to treat steel at a fundamental atomic level.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com