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Traditionally, the mechanical properties of tendons have been evaluated and reported through the use of tensile testing methodologies in vitro (Benedict et al. 1968).
Importantly, the REACH legislation states: The Commission, Member States, industry and other stakeholders should continue to contribute to the promotion of alternative test methods on an international and national level including computer supported methodologies, in vitro methodologies, as appropriate, those based on toxicogenomics, and other relevant methodologies.
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Flow cytometry was used to analyze the interaction between canine peritoneal macrophages and L. chagasi promastigotes, stained with the fluorescent dye CFSE (Fig. 1), and compared to a conventional methodology ("in vitro" binding-assays).
These intein-based methodologies permit in vitro and in vivo protein processing in ways previously not possible using traditional biochemical and genetic approaches.
The discussed ecotoxicological methodologies encompass in vitro testing (cytotoxicity, genotoxicity, mutagenicity, endocrine disruption, adaptive stress response activation, toxicogenomics) and in vivo tests (single and multi species, biomonitoring).
The methodology combines a factorial design based in the Response Surface Methodology and in vitro digestibility assays adapted to the physiology and culture conditions of the species.
Box 3. Standardization of methodology for in vitro assays aside from GIA. Standardization of qPCR methodology and modeling of data across trial centers.
This paper presents standardized methodology for in vitro assessment of bone-to-bone adhesion strength.
11 The science and methodology of in vitro fertilization (IVF) have improved considerably in the last 25 years, offering the obvious benefit of boosting the odds of getting pregnant, sometimes from almost zero.
The goal of the present study was to develop a reproducible untargeted metabolomics methodology for in vitro studies using the HepG2 human hepatocarcinoma cell line exposed to TCDD for 48 h.
AP, JK, J van D and DJ participated in the design of the study and overall discussion; CH helped to set up the methodology for in vitro metabolomics experiments; ARA optimized protocols, performed cell culture, exposures and collection of data; ARA and AL analysed data and interpreted the results.
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