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Technological limitations will be discussed so as to highlight the possibility of future improvements for new 3D-printing methodologies for tissue engineering.
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Fine needle aspiration biopsy (FNAB) cytology has been a highly effective methodology for tissue diagnosis and for various ancillary studies including molecular tests.
Freeze drying of samples is the recommended methodology for tissue cation determination as compared to "wet" methods, which exhibit lower accuracy and greater variability [ 25].
Over the last two decades, significant strides have been made in RNA profiling in FFPE tissues, including efforts to standardize tissue handling and fixation procedures [24], [25] and improving RNA extraction methodologies for FFPE tissues [13], [26], [27].
Although the Allen Human Brain Atlas and the hESC lines were processed at the same site, we note that different methodologies were used for tissue processing, and that both the microarrays and RNA-Seq for these data sets were processed off-site at different locations (Methods).
The success of the methodology for both tissue material and cell cultured material, particularly the ability to detect viral sequence after de novo assembly, is illustrated in Table 2.
In this chapter, recent advances in the experimental and computational methodologies for the bone tissue engineering scaffolds design are presented.
Bioprinting is an emerging research field boosted by the development of myriad printing methodologies for biofabrication and tissue engineering.
In this study, we demonstrated a novel methodology for aligning tissue-specific interaction networks with the yeast interactome and assess their statistical significance.
These studies introduce an efficient methodology for cataloging tissue-specific transcriptomes in which specific classes of genes or transcripts can be targeted for capture and sequence, thus reducing the significant sequencing depth normally required for accurate annotation.
The production methodology of 3D constructs for tissue regeneration is usually a complex discontinuous process involving three different stages: (1) production of 3D matrices; (2) matrix sterilisation and cell seeding; (3) in vitro dynamic cell culture.
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