Gene expression profiling was performed using the Genome U133 Plus 2.0 arrays with methodology as recommended in the GeneChip Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
Cost analyses require standardized methodology as recommended by the Panel on Cost-Effectiveness in Health and Medicine [ 2] (hereinafter, the Panel).
To study the quality of the MASQ-AD items as input of an adaptive version, we followed the methodology as recommended by the PROMIS project [ 18].
First, in order to avoid errors due to the stationary swimming, current data was obtained on competitive swimmers already familiarized with fully tethered swimming methodology, as recommended [ 8].
Parasites were isolated from these two populations using a 0.2% saponin/1 × PBS treatment and total RNA was purified, treated by RQ1 RNase-free DNase (Promega) and converted to high quality cDNAs using the SMART (switch mechanism at the 5'-end of reverse transcript) PCR cDNA synthesis methodology, as recommended (Clontech).
Several aspects, including animal care, sampling times and choice of tissues, as well as recommended endpoints and methodologies are addressed and, for each aspect, a standard procedure is proposed.
Overestimation may have occurred as a result of purposefully selecting villages to be likely to have trachoma, as recommended by the TRA methodology [12].
More precise result is expected, if the methodology is applied to more precise rate equations as recommended by the ASM models for a given case.
Five representative cities of this province, rather than six as recommended by the WHO/HAI methodology, were selected as survey areas for data collection: Nanjing, Suzhou, Yangzhou, Suqian, and Yancheng.
Our methodology is based on the UNAIDS Costing Guidelines for HIV Prevention Strategies [ 23] as recommended by Asian development bank.
