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By the mid 2000s, with three major anthologies published, and a growing literature base, there was ample scholarly literature documenting IRE across disciplines and methodologies, and subsequently, there was anecdotal data emerging from the review boards evaluating such research.
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Both these variants resulted in a 1-copy result by our real time PCR methodology and were subsequently identified by sequence analysis to be present in a heterozygous state within the SMN1 gene as evidenced by presence of the nucleotide 'C' at the critical position, c.840 that distinguishes SMN1 from SMN2.
The alternative is to spread awareness of clinical audit and to use the methodology locally at departmental level, and subsequently build on this to form a national system.
Therefore, the studies of optimization are of both scientific interest and practical implications and subsequently the methodology can potentially have many applications.
The one-time retrospective survey assumed an alternative ticketing policy for public transport, and subsequently, a methodology for simultaneously evaluating revealed-preference and stated-intentions mode choices was adopted to evaluate the willingness of commuters to adopt such a policy.
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In this study, a sophisticated statistical methodology that employs the response surface method and Monte Carlo simulation has been proposed to increase the design margin of rod internal pressure and subsequently a simplified statistical methodology has been developed to simplify the sophisticated statistical methodology.
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Initially, medium components were selected using Plackett Burman (PB) design and subsequently optimized using response surface methodology (RS M.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com