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Oligonucleotide probes (60mers) were designed to target contig sequences using the eArray Gene Expression (GE) probe design tool (Agilent Technologies, UK), employing the base composition and best probe methodologies, and designed in sense orientation with 3' bias.
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JFT and JED created the methodology and designed the experiment.
DT conceived the PMOS methodology and designed the system.
LL and MB established the PCR methodology and designed the primers.
The eArray parameters, that we used, include probe length (60nt), probes per target (1), probe orientation (sense) and design options ("best probe methodology" and "design with 3' bias").
It looks at methodologies and design goals.
Insufficient power, different methodologies, and design weaknesses explain other inconsistencies.
- Yet keywords connoting research methodologies and designs for complex systems research, as advocated in widely disseminated reviews,,,,,, do not yet generate keyword identifiers.
TRB, IFSM, MBC, JM, ENJ and EPR designed the methodology and TRB designed the statistical analysis.
AB, EPR, TRB, CV, MC, NK, and MTB designed the methodology and TRB designed the statistical analysis.
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