After describing the methodology, a series of validation studies are presented against analytical theory, experimental data, and previous numerical results.
To prove the methodology, a series of pilot experiments have been designed and performed so that human response is evaluated in a Virtual Environment (VE) reproducing the control room and a specific plant section.
Using response surface methodology, a series of experiments with three reaction factors at three levels were carried out to investigate the transesterification reaction in a microwave and conversion of alkyl ester from J. curcas oil with NaOH as the catalyst.
To demonstrate the methodology, a series of landslide hazard maps showing the probabilities of exceeding different thresholds of movement (e.g., 0.1, 0.3, and 1.0 m) were generated for western Oregon.
By applying this methodology a series of environmental indexes were obtained making it possible to quantify the impact of deposit points on the environmental elements surface water, groundwater, atmosphere, soil and human health.
In order to facilitate the application of the methodology, a series of practical indications was also introduced and collected into an "interviewer handbook".
In this methodology a series of windows with elliptical radius ranging from zero to 50% of the total population at risk was established, to ensure that both small and large clusters were found.
To ensure study fidelity to the methodology, a series of monitoring site visits were carried out by representatives of the coordinating centre (NASP), together with the two consultants from Columbia University visiting each site.
To further test the methodology, a series of artificial models based on motifs often observed in signaling systems [ 58, 59] were implemented (see Additional file 1: section S5 for model equations).
Aliquots of a single preparation of this panel were used for analysis by all of the methylation quantification methodologies in a series of independent experiments in order to test the robustness of the approaches.
