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For the analysis of specific gene families, methodological variations were introduced as reported in text.
The methodological variations were also evident for the top 20 most abundant miRNAs (Table 2).
Methodological variations were: swab placement for 10 rather than 5 seconds and rotation 360° rather than 180°.
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Whether this relates to the confounding influence of other microenvironmental parameters known to affect gene expression (e.g., low pH and low glucose), fluctuating tumor oxygenation, sampling errors caused by intratumoral heterogeneity or interlaboratory methodological variation is unclear.
Methodological variation is a potentially important cause of variation in coverage across a viral genome in different samples, but ultimately is not germane to the goals of this study, which investigated polymorphism only at those sites with sufficient coverage irrespective of why coverage may have varied among sites or across samples.
All variations were quantitative.
9 With so few studies available on the CCP/children's nutrition relationship, little can be concluded except that in Ghana at least, all three studies examining this issue have come to the same conclusion despite significant methodological variation; CCP is related to children's nutritional status, seemingly regardless of a child's sociodemographic profile.
Despite these methodological variations, our annotations are in general agreement with the other annotations, as demonstrated by a >94% overlap in the number of identified genes and a significant number of identical features, such as the number of rRNA and tRNA genes, for both completed and draft genome sequences.
Although, the present day-to-day variations are relatively small, there are some methodological considerations, which have to be mentioned.
The reasons for these variations are not entirely clear, although technical, methodological, and varying patient characteristics may partly explain these variations.
These variations are due to several factors and LCA modellers must make multiple methodological decisions during an assessment.
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