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Differences between studies are likely due to methodological variations in regards to dose, route of administration and treatment duration.
These differences may be explained by methodological variations in the in vitro models used, suggesting that HAM was more affected by the experimental conditions.
However, comparisons across the seven studies selected in this review were difficult because of methodological variations in definition of attrition, sample composition, and the factors considered potential predictors.
Methodological variations in generation of the underlying cDNA sequences, e. g. 5'-, 3'- or full length cDNA enrichment, may have led to additional bias in the datasets.
This difference could be due to methodological variations; in the case of the Jimma study, the result was confirmed based on the test result of HIV and STIs, where as in the present case, it was merely self report.
A source of variability in results not only comes from methodological variations in tissue processing (time to fixation, duration of fixation, denaturation, heating, antigen retrieval, the staining procedure) and grading scores but also from the antibody used.
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Perhaps the most difficult aspect of aggregating data from multiple sources, aside from the methodological variation in its creation, is the variation in degree of description and terms used to describe the data.
While the proliferation of different TTO specifications may be seen as a valuable and integral part of the scientific endeavor to achieve optimal valuation methods, methodological variation in TTO studies is problematic from a policy point of view.
Despite the considerable methodological variation in resilience studies, the fact that a common set of child, family, and social resources have been consistently recognised in resilience suggests that these resources are all implicated in the same underlying phenomenon, and support the validity of resilience as a construct [ 18, 23, 80].
Beauducel et al., in reporting the impact of several methodological variations used in the assessment of LDAEP, stated that, if measured reliably, P2 slopes may reflect stimulus intensity changes more precisely than N1/P2 slopes [ 25].
The results require confirmation in prospectively conducted multicentre studies with sufficient attention paid to the methodological variations common in cfDNA and DNA integrity index research before they can be incorporated into clinical decision-making algorithms.
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