The important question to consider next is why local adaptation in fitness components is absent or weak: we first consider methodological issues before turning to biological possibilities.
First, to assess the background variability due to methodological and biological factors, we averaged the values for the two LoVo replicas and compared them to the average of another two different replicates of LoVo cells, and quantified the number of genes identified as differentially expressed as a function of selected cutoff values.
An important message of our study is that consistent estimates need to be based on biology (so that estimates are not due to methodological rather than biological factors) and that studies attempting to document the start of dispersal need to be individually-based and, hence, be supported by data that allow such individuality to be estimated.
For example, authors will need to describe methodological parameters that may introduce bias or influence robustness and to provide precise characterization of key reagents, such as cell lines and antibodies, that may be subject to biological variability.
Heterogeneous results are a common feature of studies evaluating the expression and prognostic role of this gene, mainly due to both methodological and biological factors, and the prognostic impact of these two genes on DFS or OS remains to be established (Leonessa and Clarke, 2003).
To test whether the discrepancy in the steady-state tRNA abundance may be attributable to methodological or actual biological differences in tRNA regulation between diploid and aneuploid cells, we analyzed mitochondrial tRNA stoichiometry in a high-throughput tRNA sequencing dataset obtained from HEK293T cells using DM-tRNA-seq17.
The intraclass correlation has also found some interesting application in genomics where it has been used to assess methodological and biological variations in DNA microarray analysis [ 8].
The main purpose of this research article is to develop and implement a new methodological approach to characterize biological diversity using spatial model developed during the study viz.
Such an approach can estimate the overall strength and direction of any biological association in the form of an effect size by accounting for the underlying sample size, assess the degree of heterogeneity that arises among the findings of the source studies, and to statistically evaluate how methodological or biological factors shape such differences in the study results.
These patients were tested on consecutive days and the ICC and κ statistic were thus projected mainly to reflect methodological rather than biological variation.
