Important advancements in methodological molecular techniques, which permit the time- and tissue-specific inducible regulation of gene expression, will also be discussed.
The use of models allows a detailed testing of methodological assumptions in different MUNE techniques which will lead to a more accurate and reliable method of performing MUNE.
Most of these studies employed simple before after (BA) safety evaluation techniques which suffer from methodological and statistical issues.
Furthermore, we compared findings derived from different imaging techniques, which may have different methodological sensitivity.
However the adoption and extensive use of those techniques, which involve a significant number of sophisticated methodological steps, is still largely limited by the availability of usable, freely available tools gathering the specific techniques into a user-friendly environment.
More than two-thirds of studies included were of poor methodological quality in terms of sample size, variable selection, and sampling techniques, which might have resulted in some bias.
This type of design enables the collection of quantitative and qualitative data during one phase and it involves interviewing the same persons using different techniques, which in turn could help to identify measurement and methodological problems [ 26, 27].
They often use questionable techniques which backfire.
Among the publications that did not proffer checklists by which to determine quality, methodological techniques signalling quality were also prominent among the authors' recommendations [ 26, 40, 44, 49].
There are common methodological problems with each technique, which may be categorised under the headings measuring probe error, pressure measurement volume displacement error and volume aliquot inflation error.
