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It seems, therefore, that the detection of genetic variation for DA in this genus appears to be basically a methodological problem, including statistical power and the environmental conditions where the experiments are performed.
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In general, available studies still suffer from methodological problems including small sample size, limitations of retrospective chart review, lack of standardized measures, overemphasis on inpatients, and lack of longitudinal data.
Human imaging studies of DAT availability in relation to chronic tobacco smoking are sparse and have generated conflicting results [28, 33] possibly related to methodological problems including the choice of radioligand and sample size.
Prior studies evaluating predictors of pain-related outcomes following treatment for sciatica have been limited by methodological problems, including retrospective study design, use of unvalidated outcome measures, and short-term follow-up periods.
With a few exceptions, the delinquent literature suffers from methodological problems, including biased sampling methods, small numbers of subjects, failing to objectively diagnose conduct disorder, incorrect use of specific statistical procedures, and lacking of appropriate control groups.
Gaps in knowledge are attributed to specific conceptual and methodological problems, including the lack of basic knowledge about pain behavior, over-reliance on an invasive short-term pain model, pain measurement issues, and lack of knowledge about confounders.
Generally, cluster randomized trials, are susceptible to a range of methodological problems including selection bias [ 18].
Also, there was significant publication bias and other influential methodological problems including with self-rating, control condition and imputation.
Second, conclusions from the study of creatine supplementation in several myopathic conditions are deeply hampered by methodological problems including difficulties in statistical power due to rarity of diseases.
However, the studies were somewhat limited by methodological problems including small sample size and the use of assessment tools of limited validity [ 13].
These discrepancies may be due to methodological problems, including relatively small numbers of cases and controls and selection of control populations [ 44].
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