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Reviewer #2: 1) My primary concern is that there is insufficient methodological detail for the quantification of c-Fos + cells.
The quality of the reviews varied and the reporting often lacked sufficient methodological detail for quality assessment.
The integration of behavior, electrophysiology, and immunohistological data provide convincing evidence that IL suppression through D1 activity is necessary for reinstatement. 1) My primary concern is that there is insufficient methodological detail for the quantification of c-Fos+ cells.
Studies undertaken as single studies rather than as part of a programme, initiative or fellowship were included because detailed summaries were available for the former whereas summaries were not available or had no methodological detail for latter types of studies.
However, this was only possible with the cooperation of H-C&C, as the original paper did not include the necessary methodological detail: for example, the Read and other codes used to define IHD and other morbidities; how drug exposures were measured; the precise specification of each covariate; and the method used to select matched controls.
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Methodological details for the neophobia, consumer innovation and technical innovation tests are presented next.
Further methodological details for delineation of the Cg1, PL, and IL borders were described in Suppl.
Full methodological details for the IHS are provided elsewhere [ 23].
Methodological details for assessing gp120-associated neurite degeneration are available in Supplementary material.
The complete methodological details for the latter library preparation protocol will appear in a separate manuscript that is currently in preparation for publication.
This study has the following key implications: First, we provide the methodological details for calculating YLL to measure the burden of premature mortality for any geographic area that has death registry data.
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