The use of non-chromatographic methods would overcome these drawbacks and hence we investigated acid pH treatment and ammonium sulphate precipitation as methods for SELP purification.
Establishing these culture methods would overcome the foreseeable barrier of obtaining enough pure, donor spermatogonia from a minimally invasive testis biopsy to effectively restore a patient's fertility.
Moreover, performance-based methods would overcome another methodological issue related to self- and/or informant-report measures.
At this point, use of a semi-supervised method would have overcome the deficit.
In OK+M+S method, since Sox2 was infected at least, Oct4 would overcome the counteractions from Klf4, which led to temporary up-regulation of Snail2 and impaired up-regulation of E-cadherin.
Nevertheless, the use of methods such as domain decomposition, combined with parallel computing, would overcome this difficulty.
However, we felt that including all 6, followed by vote taking would overcome any weakness in any single prediction method.
As the devices in the communication links are synchronized through GPS time stamping [81], the accuracy of the time synchronization would be 1 μs and would overcome the challenge of the current differential protection method.
(7– 9) A simple, noninvasive method to determine CRAd distribution and to accurately measure CRAd replication in vivo would overcome this limitation.
Changing the gear material to a stainless steel would overcome this issue and also open up alternative manufacturing methods such as hobbing or wire EDM.
