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GO was synthesized using the Hummers method with minor revisions as previously described [18].
The magnetite nanoparticles (MNPs) were prepared by the conventional co-precipitation method with minor modifications [30].
SILAC labeling was carried out according to a previously published method with minor modifications (Zhou et al., 2010).
The AgNPs were prepared according to the previous reported method with minor modification (Fan et al. 2009).
For flavonoid extraction, flavonoids were extracted using an ultrasonic-assisted method with minor modifications (Huang et al. 2009).
The PICO TAG method, with minor modifications, was used for measuring the amino acid profile of the hydrolysate [12].
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The binding of σ ligands to σ1 and σ2 receptors was determined according to literature methods with minor modification [50].
Killing experiments of SS2 by pig PMNs were carried out as described in earlier methods with minor modifications [60], [61].
Primary cultures of rat hippocampal neurons were prepared according to previously described methods with minor modifications [47].
Bet v 1.0401 batch A was produced according to previously published methods with minor modifications [12], [13], [14].
To evaluate and optimize methods for early molecular diagnosis of P. falciparum malaria in challenge trials, RTQ-PCR was performed on 0.5 mL of venous blood collected contemporaneously with blood smears using published methods with minor adaptation [23].
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