Another popular method with decreased reproducibility is cell disruption by manual grinding in mortar with sterile sand.
Then each candidate is tested using different methods with decreasing stringency to assign them to the most probable known effector gene (see Methods section).
Although, as expected, the performance of every method declined with decreasing dataset size, the magnitude and timing of this decline varied strongly per method.
The common characteristic of test results is that porosity indexes obtained by various methods decrease with decreasing corrosivity of the test process, and approach constant values.
We did observe temporal changes in HCC diagnostic methods with decreased reliance on liver biopsy.
Starting from the fact that open heart surgery can be performed in a patient with a lower risk using standard methods with decreased morbidity and mortality rates, it can be conceived that more valuable gains can be obtained in patients with a higher risk.
It has been widely reported that the efficiency of these methods critically improves with decreasing salinity and increasing pH of the slug injected during LSW and alkaline flooding respectively, and greater amount of oil can be recovered in these conditions.
The structure was further refined by EMAN1 using standard projection matching method with progressively decreasing angular step size (with a final value of 0.4°).
The fit-algorithm minimizes the sum of least squares between the calculated and measured Δ-values, SSQ = ∑ i w i ⋅ (Δ i, 0 (R i ) − Δ i, 0 hook ) 2, by adjusting the parameters αc, βc and Σc(0) using an iterative gradient method with linearly decreasing step size.
On the other hand, the performance of several network inference based methods, such as Genomica (iterative NI method A) and GENIE3 (direct NI method A), remained relatively stable with decreasing number of samples.
