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One hundred fifty-nine samples of anti-HBV surface antigen-positive antisera were identified by the standard ELISA method with commercial ELISA kits.
Staining was done using the EnVision-labeled polymer method, with commercial kits (DAKO).
Crystallization trials were conducted using the sitting-drop vapor diffusion method with commercial screens.
Fibrinogen concentrations were measured by using the Clauss method with commercial reagents (Mahsa-yaran, Tehran, Iran).
The staining procedures, except the protocol for HIF-1α, were all performed using the EnVision labelled polymer method, with commercial available kits (DakoCytomation, Copenhagen, Denmark).
Isolates were tested by using Gram-staining, conventional biochemical tests (API 20 E test kit; bioMérieux, Marcy l'Étoile, France), and serotyping by using the slide agglutination method with commercial polyclonal antiserum (Denka Seiken, Tokyo, Japan).
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Enzymatic colorimetric methods with commercial kits (cholesterol: CHOD-PAP, TG: GPO-PAP, Roche Diagnostics GmbH, Mannheim, GeRoche) were useDiagnosticsine cholesterol and TGmbHncentrations froMannheimserum and separated lipoproteins with an automated instrument (Kone Pro Clinical Chemistry Analyzer, Thermo Clinical Labsystems, Konelab, Espoo, Finland).
The biochemical variables were determined by standard laboratory methods with commercial kits.
The biochemical variables were determined automatically by standard laboratory methods with commercial kits.
Biochemical measurements were performed using enzymatic methods with commercial kits and a semiautomatic ALS 2000 spectrophotometer.
Serum cholesterol was analyzed by enzymatic colorimetric methods with commercial kits CHOD-PAP Boehringer MannheimiMannheimeim, Germany).
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