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Phylogenetic tree was constructed using neighbor-joining method with bootstrap (1000 replicates) by Kimura 2-parameter model using MEGA 5.1 program.
Phylogenetic trees were constructed using the MEGA 6.0 software program (Tamura et al. 2013), based on representative sequence for each OTU/aOTU, by neighbor-joining (NJ) method with bootstrap values calculated from 1000 replications.
ClustalW [93] multiple protein alignments were used to construct phylogenetic trees as determined by the Neighbor Joining method with bootstrap re-sampling (detailed in the legends for Figures 1), using MEGA3.1 [94].
The alignments files generated were then submitted to the graphical interface of SeaView version 4 software [40] to construct phylogenetic trees using the neighbor-joining method with bootstrap of 1000 replications.
maximum likelihood method with bootstrap replicates set as 1000.
The neighborhood joining method, with bootstrap values of 2000, was utilized to conduct the phylogeny test.
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To answer both questions, we used the support vector machines (SVMs) method with bootstrapping.
A support tree-clustering method with bootstrapping using expression data was performed to statistically validate the tool.
Phylogenetic trees were constructed in MEGA4 through the Neighbor-joining (NJ) method with bootstrapping test (1000 replicates).
This means that Method 3, which is the method with bootstrapping and genotype optimisation, gave the best results for all values of noise.
All phylogenetic analyses were performed with MEGA v5.05 (Tamura et al. 2011) using maximum-likelihood (ML) methods with bootstrap values from 10,000 replicates.
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