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Compared with classical molecular biology protocols and cytogenetic analyses used as reference standards, results of the 26-plex RT-PCR method were concordant in all 204 (100%) cases of adult AML tested.
This high level of concordance mirrors that observed in our previous analysis of X-linked gene expression in TSCs, where allelic biases determined via RNA-Seq and an alternate method were concordant in 18 of 18 assays (nine genes in two TSC lines) (Calabrese et al. 2012).
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This method was concordant with our more general approach to identify the number of genes sampled, where we tested for sequence matches across several databases, including the Tribolium genome (Table 2).
On the other hand, when PSB was performed without antibiotics, direct examination was positive in 65% (n = 46), culture was positive in 62% (n = 43) and those methods were concordant, non concordant and partially concordant in 71% (n = 50), 18% (n = 12) and 11% (n = 8), respectively.
When PSB was performed under antibiotic treatment, direct examination was positive in 26% (n = 12), culture was positive in 28% (n = 13) and those methods were concordant, non concordant and partially concordant in 93% (n = 43), 4% (n = 2) and 3% (n = 1), respectively.
The estimates obtained from both methods were concordant.
Results obtained from both methods were concordant in 183 (82.8%) cases (κ=0.48).
In 433 patients (86%), however, the two methods were concordant in weighting the "other risk factors and disease history" with a weighted κ value of 0.764.
In 9 out of the 12 responders (75%), the results of the LV d P/d tmax and PEAarea methods were concordant (Table 2 ).
When comparing DNA tetraploid gastric cancers combined with DNA diploid gastric cancers with DNA aneuploid gastric cancers (category definition C), results from both methods were concordant in 174 (78.7%) gastric cancers (χ P<0.001, κ=0.46; Table 2C and E).
When comparing DNA diploid with DNA aneuploid gastric cancers (category definition A), results from both methods were concordant in 183 (83%) gastric cancers (χ P<0.001, κ=0.48; Table 2A and E).
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