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To demonstrate the versatility of our method, we targeted QDs to cell surface cyan fluorescent protein and epidermal growth factor receptor in HeLa cells and to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors in neurons.
Using this method, we targeted a FDR of 5% and probe sets with q-values below 0.05 are considered to be of interest.
To test the limits of spatial selectivity of our method, we targeted head neurons in L2 larvae.
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With regard to the discussion of missing data estimation methods, we targeted the application of previously-published imputation or estimation methods.
Using either delivery method, we achieved targeted sequence integration at high efficiency (up to 40%) via homology-directed repair.
By applying social network analysis techniques to data collected using the Dual Perspectives Method, we identified targeted systems-based interventions that may reduce unnecessary interruptions while avoiding unintended consequences that impose additional burden on ICU staff.
The MPSS method we used targeted the 3′-most occurrence of the DpnII recognition sequence, 5′-GATC-3′.
With this new method, "we can target specific cells, turning them off and on, and so we're able to really see how this is all wired up".
To expand our findings on the cell cycle synchronization method to other cell types, we targeted the EMX1 gene in human primary neonatal fibroblasts (neoFB) and H9 human embryonic stem (hES) cells.
In this method, we create target databases by 'subtracting' higher node sequences from a lower node database and then we 'search' human domains against them for detecting remote homologs – so we use the term 'subtractive searching' method for this type of tracing process.
Because we sampled with a Remotely Operated Vehicle (ROV) (see Methods), we could target visible zones where the halocline impinged the seafloor (Fig. 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com