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As a modification to the standard method, we limited ourselves to a qualitative rather than quantitative analysis, as our study did not attempt to quantify the impact of each emerging theme on the potential impact of interdisciplinary communication from a systems perspective.
In regard to the stationary requirement for applying this method, we limited our analysis to the three first NREM-REM cycles [ 42].
Based on the ADR detection method, we limited the inclusion of studies to those that used intensive chart review or voluntary reporting by healthcare professionals combined with measures to stimulate voluntary reports.
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Given the vast number of potential normalization and preprocessing methods, we limited our exploration to methods for which there is a rationale.
Because of the time-consuming training required for supervised methods, we limited our evaluation to networks with 30 nodes extracted from E. coli networks.
Following further editing and transcript quality assessment (see Methods), we limited our dataset to 118,258 transcripts belonging to 82,331 loci (N50: 2,092; mean length: 1,352 nucleotides).
As described in Materials and Methods, we limited our PCR amplicons to 100 bp, and performed pilot experiments to confirm similar expression in matched fresh-frozen and FFPE tumours (data not shown).
For both methods, we limit the search to sounds shorter than 20 seconds in order to filter out longer field recordings.
To be compliant with our system, we introduced two small variants into the original method: first, we limited the size of є to a maximum of 8×8 also for those keypoints with larger spatial support; second, we used the same weighting window of Equation 3 to replace the original neighborhoods.
Resulting p values were adjusted for multiple comparisons by the Benjamini and Hochberg [ 44] FDR method, and we limited statistically significant sites to those that passed an FDR <5%%.
In our implementation of the method for ESPRESSO, we limited the number of loci to two because we generate only two SNPs in LD.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com