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By using the SDIA method, we induced dopaminergic (DA) neurons by coculturing HLA-G1-H1 hES cells with the mouse stromal cell line PA6.
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The simple method we used to induce BirA-driven high efficient biotinylation was effective for a number of different secretory and membrane bound proteins, with the BAP tag positioned both at the C- and N-terminus.
Conclusion: Using a reliable method, we demonstrated that nVNS induces a rapid onset analgesia in healthy subjects.
Through our unique method, we are able to induce laminar flow within intact mouse aortic tissue and illicit subsequent cellular responses.
In addition, a new subsection titled "Reconstitution of de novo centriole formation" is added to the Materials and methods, describing how we induce de novo centriole formation in clonal or non-clonal cell populations.
In the present work, using two different methods, we conclude that artemisinin induced apoptosis in P815 cells and not in BSR cells.
On the basis of previous work by ourselves and others (13– 15; see also Materials and methods), we initially studied adhesion induced by 100 U/mL of TNF-α alone, or combined with 10 ng/mL of IFN-γ.
Our results show that the mechanism of protection does not involve inhibition of cysteine proteases such as cathepsin B. Using multiple methods, we demonstrate that CysC induces autophagy in cells under basal conditions, and enhances the autophagic activation in cells exposed to nutritional deprivation and oxidative stress.
To address these deficiencies, we propose a new class of constraint-aggregation method that we call induced aggregates.
With this combination of methods we found that etoposide mostly induces SSBs, and that only a subset of the DSBs activates a DNA-damage response and cell death.
"Using a method called Induced Polarization (IP) survey, we lay out extensive wires in shallow pits across this vast area.
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CEO of Professional Science Editing for Scientists @ prosciediting.com