To determine the solution structure of the core SMN Gemin2 complex using heteronuclear NMR methods, we expressed and purified N- and C-enriched SMN26 51 and Gemin295 280 and obtained comprehensive resonance assignments using triple resonance, total correlation spectroscopy and NOE-based strategies (see the Materials and methods section).
According to the mode analysis method, we can express the solution to (12) as the sum of different modes [40] as wleft {z,s} right) = sumlimits_{i=1}^{infty} {varphi_i left z right)q_i left(s right)}, (22 where ϕ i (z) is the beam shape for the ith mode and q i (s) is the corresponding generalized coordinate.
In order to repeat the main construction step of the above mentioned graph entropy method, we first express some mathematical preliminaries [71], [72], [28].
Based on the three-dimensional field equations and the traction-free boundary conditions, by using a coupled series-asymptotic expansion method, we manage to express the total elastic potential energy of the cylinder in terms of the leading order term of the axial strain.
By combined these methods, we sucessfully expressed active rh-bikunin at the high level.
To select differentially expressed genes, we adjusted for multiple testing using the False Discovery Rate (FDR) method; we report as differentially expressed genes with FDR adjusted p-value < 0.15.
Using a deep-sequencing method, we identified 367 differentially expressed miRNAs (including 46 conserved miRNAs and 321 novel miRNAs) and compared their expression levels in the two genotypes.
We applied this method to express an endoplasmic reticulum-green fluorescent protein (ER-GFP) fusion protein in P. tenuiflora, a grass halophyte with extreme tolerance to alkaline soils (pH ≈ 10).
To establish such a method we used a HeLa clone expressing the core histone H2B fused to the enhanced yellow fluorescent protein (H2B-EYFP).
To substantiate these observations by a further method, we generated transgenic parasites episomally expressing PfPV1 fused to a GFP reporter, under control of the PfCRT promoter.
