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Although the study restricts attention to the maximum likelihood (ML) fitting method, we anticipate that the drawn recommendations would apply to other fitting methods also.
Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects.
Given the substantial number of TF tissue associations recovered by our method, we anticipate that PASTAA could also be applied directly to a non-ranked group of genes acting in a different functional context such as a metabolic pathway.
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From previous trials with similar follow-up methods we anticipate, conservatively, that 80% of patients randomised will complete a follow-up questionnaire at 26 weeks [ 18].
Furthermore, given that this study only focused on the subset of "similar to" cDNA clones and did not cover those annotated as "weakly similar to" (see methods) we anticipate that there are many more pathologs in the mouse that are yet to be identified.
Additionally, while short-acting methods currently dominate the method mix in most countries, as the international community focuses on expanding long-acting method options, we anticipate that their contributions to the CPR will increase substantially.
Our newly designed primers based on 66 nymphalids also provide a basis for sequencing additional butterfly mitogenomes by the Sanger method, while we anticipate that pyrosequencing will become prevalent for mitogenomic studies.
The performance of the proposed methods is comparable to contemporary methods, and we anticipate that careful examination of the relation between the log-spectral domain and the embedded domain might lead to further improvements.
Periodic assessment of metrics for each approach will allow WormBase curators to fine-tune these methods as we anticipate that both the content of the published literature and curation priorities may change.
As sequencing methods continue to improve, we anticipate that this approach could be a valuable addition to preimplantation testing.
Although we have not compared our method directly with these constrained docking methods, we would anticipate that the enrichments seen would be quite similar (if not better with constrained docking because the conformational search done by the docking program is biased in favor of solutions that fit the constraints).
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