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To conform all hPSC lines to one universal culture method, we adapted all lines to feeder-free monolayer growth [28] on the basement membrane matrix Geltrex in conditioned medium [29].
For our main analysis method, we adapted spectral analysis ([52], [53], see full description in methods section) to extract the correlation coefficient of each voxel's response to a model of the auditory stimulus repetition frequency and its phase (see Fig. 1).
For one method, we adapted a standard screening protocol to directly identify plaque-forming units (PFUs) or "free" phage particles within >150 different environmental extracts from an array of soil, fresh water and marine samples, as well as herbivore feces, rhizosphere and phylloplane washings, decaying organic matter, and soil invertebrate intestines.
To establish a flexible, cost-effective, high-throughput genotyping method, we adapted SNPlex genotyping established and systemically validated by Applied Biosystems to have high precision [ 18, 19].
For the second method, we adapted the sandwich approach by Samy et al., where temperature was measured on a thin film of PDMS located about 150 μm below the iDEP channel.
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The method we adapt is based on the work of Itti et al. [8].
The first solution method we adapt to the requirements of our problem is a dynamic variable neighborhood search (VNS), presented for the DSDARP by Schilde et al. (2011).
To improve sensitivity and efficiency of radiation hybrid panel analysis in comparison to gel-based methods, we adapted fluorescence-based real-time PCR and dissociation curve analysis for use as a novel scoring method.
We extracted DNA using a commercial kit (DNeasy Blood and Tissue Kit, Qiagen) and measured the mean telomere lengths in WBC, splenocytes and hepatocytes using a real-time PCR method [28] we adapted for wild mice [5].
Here we describe the methods by which we adapted the relevant human psychophysical methods to awake, behaving monkeys and replicated key previous psychophysical results.
Given the potential to use this method for high-throughput DNA extraction, we adapted the method for cyanophage.
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